Biology - Molecular Biology In Medicinal Chemistry by Thomas Lengauer, Raimund Mannhold, Hugo Kubinyi, Hendrik

By Thomas Lengauer, Raimund Mannhold, Hugo Kubinyi, Hendrik Timmerman

Offers a huge, application-oriented evaluation of this expertise. The state of the art in bioinformatics is evaluated both from an international view via introducing actual program eventualities.

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HeLa cells were stimulated with 1 ng/ml IL-1a for the indicated times at 37 C. 2) are shown. NucCytoInten ¼ nuclear minus cytoplasmic fluorescence intensity. Fig. 3 Protein–GFP Fusions Tagging technologies have become popular to monitor the movement or transport of molecules into cells and within cells. Extracellular ligands can be conjugated to biotin and later visualized with enzyme-conjugated streptavidin. A HTS assay has been described on this basis for epidermal growth factor (EGF) internalization [97].

3 Gene and Protein Expression Profiling in High-throughput Formats monitored, the novel techniques directly monitor gene expression by quantitation of the mRNA levels for several to up to thousands of genes simultaneously. Furthermore, these techniques do not require the prior introduction of a detection system, allowing unlimited choice with respect to cell type or tissue derived from organs. 3 gives an overview of a number of techniques developed for HTS applications that are currently available.

More details including other well-established ion-channel assay technologies were described in a recent review article [34]. FLIPR Technology and Ion Channel Assays To identify compounds that modulate ion channel activity, it is imperative that rapid and economical evaluation of biological activities in a high-throughput manner is available. One of the fluorescencebased assays of membrane potential changes uses a potentiometric fluorescent probe, bis-(1,3-dibutylbarbituric acid) trimethine oxonol [DiBAC4 (3)], that partitions between the intracellular and extracellular compartments depending on the electropotential across the membrane.

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